Antioxidant Activity and Potential Bioactive Peptides from Skin Protein Hydrolysate of Yellowfin Tuna (<I>Thunnus albacares</I>)

Highlight Research Immersion of tuna skin using NaHCO3 at low temperatures effectively reduces the fat content. The DH value, MW, and amino acid composition are in line with antioxidant activity hydrolysate. ABTS method showed highest FPH shows functional group characteristics that similar to collagen hydrolysate products. has potential as a source antioxidants, ACE inhibitors, antifibrinolytics. Abstract fillet industry produces abundant by-products high protein, which raw material for fish protein (FPH) bioactive peptides. Exploration peptides from is generally hydrolyzed gelatin collagen. study aimed produce directly an identify research began by defatting multiple concentrations immersion times. defatted was produced enzymatic hydrolysis different papain enzyme selected treatments were assessed results process 0.50% 30 minutes generated reducing content value 80.53%. Using gave significant effect on FPH, 29.72-67.64%. Therefore, obtained 4 hours chosen characterize peptide. Hydrolysis 5% DPPH, ABTS, power values 0.965 mg/mL, 0.495 0.415 absorbances, respectively. possesses molecular weight 10.15-48.50 kDa. Functional groups detected amides A, B, I, II, III. Glycine, proline, arginine became acids dominant FPH. Based diversity biopeptide compounds, several biological function candidates detected, namely antifibrinolytics have be used nutraceutical